Our lab is highly experienced at purifying proteins, especially members of the cytochrome P450 super family of enzymes, and studying their regulation and function in metabolism of steroids, xenobiotics and activation of chemicals to carcinogens. We have produced an extensive collection of over 70 antibodies specific for individual P450 isozymes and used these as probes of individual P450 enzymes to study their tissue distribution, their topology in membranes, the mechanisms of inhibition of catalysis by certain monoclonal antibodies and the contribution of individual P450 enzymes to drug and xenobiotic toxicity. More recently, we have sought to understand the mechanisms underlying the unique sensitivity of the female ACI rat (compared with other strains) to induction of mammary cancer in a relatively short time by exposure to exogenous estrogen alone. It is possible that the female ACI rat could serve as a useful model for human breast cancer, which could be used to test environmental factors that may decrease or increase risk of breast cancer. I am also the director of the NIEHS/EOHSI/CINJ Proteomics Facility, where state-of-the-art protein detection methods are used in 2D gels to examine changes of the proteome during carcinogenesis to uncover biomarkers of cancer progression and characteristics of a cancer that could be exploited to stop its growth. Other investigators use the Facility to examine changes in various proteomes in order to understand the mechanism of action of certain toxic environmental chemicals.

	The Future of Proteomics in the Study of Alcoholism. Kasinathan, C., Vrana, K., Beretta, L., Thomas, P.E. , Gooch, R., Worst, T., Walker, S., Xu, A., Pierre, P., Green, H., Grant, K., Manowitz, P. . Alcohol Clin Exp Res. 2004 28(2):228-232 .
	Identification of UGT2B9*2 and UGT2B33 isolated from female rhesus monkey liver. Dean B, Arison B, Chang S, Thomas PE, King C. Arch Biochem Biophys. 2004 Jun 1;426(1):55-62.
	The effects of steroidal estrogens in ACI rat mammary carcinogenesis: 17ß-estadiol, 2-hydroxyestradiol, 4-hydroxyestradiol, 16a-hydroxyestradiol, and 4-hydroxyestrone. Turan, V.K., Sanchez, R.I., Li, J.J., Li, S.A., Reuhl, K.R., Thomas, P.E. , Conney, A.H., Gallo, M., Kauffman, F.C., Mesia-Vela, S. Jour of Endocrinology, 2004 183(1):91-99.
	A novel proteomic coculture model of prostate cancer cell growth. Dvorzhinski D, Thalasila, A, Thomas, PE, Nelson, D, Li, H, White, E, DiPaola RS, Proteomics-Journal 2004 4: 3268-3275